Description
Our bioactive FGF-1 (FGF-acidic) Human, E. coli Recombinant Protein, also known as Fibroblast Growth Factor 1, is a single, non-glycosylated, polypeptide chain that contains 140 amino acids and has a molecular mass of approximately 15.8 kDa. The FGF-1 is purified by proprietary chromatographic techniques.
Summary
FGF-acidic, a member of the fibroblast growth factor (FGF) family, is a non-glycosylated heparin binding growth factor encoded by the FGF1 gene. FGF-acidic plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro. Acts as a ligand for FGFR1 and integrins. Binds to FGFR1 in the presence of heparin leading to FGFR1 dimerization and activation via sequential autophosphorylation on tyrosine residues which act as docking sites for interacting proteins, leading to the activation of several signaling cascades. Binds to integrins. Its binding to integrins and subsequent ternary complex formation with integrins and FGFR1 are essential for FGF1 signaling.
Other Names
Fibroblast Growth Factor-1, FGF1, FGF-1, FGF acidic, FGF-a, Acidic fibroblast growth factor, aFGF, Heparin-binding growth factor 1, HBGF-1.
Amino Acid Sequence
MFNLPPGNYK KPKLLYCSNG GHFLRILPDG TVDGTRDRSD QHIQLQLSAE SVGEVYIKST ETGQYLAMDT DGLLYGSQTP NEECLFLERL EENHYNTYIS KKHAEKNWFV GLKKNGSCKR GPRTHYGQKA ILFLPLPVSS D.
Source
E. coli
Formulation
Sterile filtered white lyophilized (freeze-dried) powder. The protein was lyophilized from a concentrated (1 mg/ml) sterile solution containing PBS, pH 7.4.
Purity
Greater than 95.0% as determined by:
- Analysis by RP-HPLC.
- Analysis by SDS-PAGE.
Biological Activity
The ED50, calculated by the dose-dependent proliferation of mouse BALB/c 3T3 cells is <0.5 ng/ml, corresponding to a specific activity of > 2,000,000 IU/mg.
Reconstitution
It is recommended to reconstitute the lyophilized FGF-acidic in sterile 18MΩ-cm H2O at 4°C at a concentration of 0.1 - 0.25 mg/ml. Allow sample to sit for 5 min. at 4°C, spin to remove precipitant.
Shipping
Ships at ambient temperature. Upon receipt, store the product at the temperature recommended below.
Storage/Expiration
Lyophilized FGF-acidic, although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution, FGF-acidic should be stored at 4°C between 2-7 days and for future use below -18°C. Please prevent freeze-thaw cycles.
Usage
This product is intended for Laboratory Research Use Only. Not for use in diagnostic or therapeutic procedures. This product may not be used as a pharmaceutical or veterinary drug, agricultural product, or food additive.
Resources
Want to learn more about FGF-acidic? We have compiled the links below which contain information that you may find interesting.
- UniProt Knowledgebase: UniProtKB - C9JDC5 (C9JDC5_HUMAN): Fibroblast growth factor 1
- National Library of Medicine: Gene ID: 2246: FGF1 fibroblast growth factor 1 [Homo sapiens (human)]