Description
Quantify both endogenous active MMP-2 and total MMP-2
The QuickZyme Human MMP-2 Activity Assay enables you to specifically measure in biological samples both active MMP-2, as well as (pro)MMP-2, which is activated on the plate by APMA. It can be used for the measurement of MMP-2 activity in various biological samples, such as conditioned culture media, tissue homogenates, serum, plasma and urine.
This 96-well plate format assay is based on the QuickZyme technology, using a modified pro-enzyme as a substrate, which upon activation is able to release color from a chromogenic peptide substrate. This multiplication step provides a unique assay sensitivity.
Features
- Measures both endogenous active MMP-2 (naturally occurring) and total active MMP-2 (following activation with APMA).
- Samples: cell culture conditioned medium, serum, plasma, urine and tissue homogenates.
- Quantitative.
- Range: 0.02 – 16 ng/ml.
- Sensitivity: 0.04 ng/ml for 2 h incubation with detection reagent; 0.02 ng/ml for 6 h incubation with detection reagent; 4 pg/ml for overnight incubation
- Ease-of-use: Equivalent to ELISA.
Type
Enzyme activity immunoassay
Applications
- cell culture conditioned medium, serum, plasma, urine and tissue homogenates
Sensitivity
- 0.04 ng/ml (2 hr incubation)
- 0.02 ng/ml (6 hr incubation)
- 4 pg/ml (o/n incubation)
Quantitative Measurement
Typical data with 4 hours incubation.
Storage
Unopened kit: Store at -20°C, except for the standard, this vial should be stored at -70°C. Do not use kit, or individual kit components past kit expiration date.
Opened kit / reconstituted reagents: Please refer to kit manual.
Other names
Matrix metalloproteinase-2, Gelatinase A, TBE-1, 72 kDa type IV collagenase, 72 kDa gelatinase, Gelatinase A, MMP-2, TBE-1, PEX, MMP2 CLG4A
Resources
Product References
- Ancona, Silvia et al. “Differential Modulation of Matrix Metalloproteinases-2 and -7 in LAM/TSC Cells.” Biomedicines vol. 9,12 1760. 24 Nov. 2021, doi:10.3390/biomedicines9121760
- Ntika, Stelia et al. “Elevated Glucagon-like Peptide-1 and a Th2 Shift May Support Reduced Prevalence of Thoracic Aortic Aneurysm in Patients with Diabetes.” Journal of cardiovascular development and disease vol. 8,11 143. 28 Oct. 2021, doi:10.3390/jcdd8110143
- Ripmeester, Ellen G J et al. “BMP7 reduces the fibrocartilage chondrocyte phenotype.” Scientific reports vol. 11,1 19663. 4 Oct. 2021, doi:10.1038/s41598-021-99096-0
- Ntika, Stelia et al. “Syndecan-1 Expression Is Increased in the Aortic Wall of Patients with Type 2 Diabetes but Is Unrelated to Elevated Fasting Plasma Glucagon-Like Peptide-1.” Biomedicines vol. 9,6 697. 20 Jun. 2021, doi:10.3390/biomedicines9060697
- Shiroto, Yuki et al. “Matrix Metalloproteinase-2 Activated by Ultraviolet-B Degrades Human Ciliary Zonules In Vitro.” Acta histochemica et cytochemica vol. 54,1 (2021): 1-9. doi:10.1267/ahc.20-00021
- Wu, Wen-Chi et al. “Do different bariatric surgical procedures influence plasma levels of matrix metalloproteinase-2, -7, and -9 among patients with type 2 diabetes mellitus?.” World journal of diabetes vol. 11,6 (2020): 252-260. doi:10.4239/wjd.v11.i6.252
- Ruan, Jun Shan et al. “Ursolic Acid Attenuates TGF-β1-Induced Epithelial-Mesenchymal Transition in NSCLC by Targeting Integrin αVβ5/MMPs Signaling.” Oncology research vol. 27,5 (2019): 593-600. doi:10.3727/096504017X15051723858706
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Ilex Life Sciences LLC is an official distributor of QuickZyme Biosciences.