Description
Enzyme immunoassay for the quantitative determination of Anti-Müllerian Hormone (AMH) in human serum and plasma samples. Quality controls are included with the kit. Developed and manufactured in Europe by PromedeusLab.
Summary
Anti-Müllerian Hormone (AMH) is a glycoprotein belonging to the transforming growth factors (TGF-P). In females, AMH is secreted by granulosa cells of small follicles in the ovary (1). Serum AMH levels strongly correlate with the number of growing follicles. Serum AMH levels are used in individualized follicle-stimulating hormone dosing protocols and may predict the risk of poor response or ovarian hyperstimulation syndrome (3). Serum concentrations of AMH gradually decrease and fall below detectable levels in menopause. AMH is the best current available measure of ovarian reserve for different clinical conditions (2).
Other Names
Anti-Müllerian hormone, Muellerian-inhibiting factor (MIF), Muellerian-inhibiting hormone (MIH), Muellerian-inhibiting substance (MIS), UniProtKB# P03971
Principle of Method
The microtiter plate is coated with the antibody specifically binding the Anti-Müllerian Hormone. The human serum or plasma is incubated in the plate with the capture antibody.
The specimen is washed out and the specifically bound protein is incubated with biotin-labelled detection antibody. Following another washing step, Streptavidin-HRP conjugate is added into the well. Unbound reagent is then washed out. Horseradish peroxidase (HRP) bound in the complex reacts with the chromogenic substrate (TMB) creating the blue colour. The reaction is stopped by addition of STOP solution (H2SO4).
The absorbance values are measured at 450 nm (optionally 450/630 nm) and are proportional to the concentration of AMH in the specimen. The concentration of AMH in unknown samples is determined from the calibration curve which is created by plotting the absorbance values against the standard concentration values.
Sample Types
Serum, Plasma
Typical Standard Curve
Assay Range: 0.094 ng/mL - 3 ng/mL
Sensitivity
The limit of detection, defined as a concentration of human AMH giving absorbance higher than absorbance of blank + 3 standard deviations, is better than 0.024 ng/mL of sample.
Precision
Intra-Assay
| Test sample | Mean (ng/mL) | SD | CV |
| 1 |
1.9 | 0.1 | 7% |
| 2 |
3.4 |
0.2 | 5% |
Inter-Assay (Run-to-Run)
| Test sample | Mean (ng/mL) | SD | CV |
| 1 |
2.0 | 0.1 |
4% |
| 2 |
3.7 |
0.2 | 6% |
Accuracy
Dilution Linearity:
| Test sample | Dilution |
Measured Concentration (ng/mL) |
Expected Concentration (ng/mL) |
Yield |
| 1 |
1.9 |
- | - | |
| 1 | 2x | 1.1 | 0.96 | 110% |
| 1 | 4x | 0.5 | 0.5 | 104% |
| 1 | 8x | 0.3 | 0.2 | 109% |
| 2 |
3.4 |
- |
- | |
| 2 | 2x | 1.8 | 1.7 | 108% |
| 2 | 4x | 0.9 | 0.9 | 108% |
| 2 | 8x | 0.5 | 0.4 | 113% |
Spiking Recovery:
| Test sample | Spike (ng/mL) |
Measured Concentration (ng/mL) |
Expected Concentration (ng/mL) |
Yield |
| 1 |
- | 1.2 |
- | - |
| 1 | 3.0 |
4.0 |
4.23 |
95% |
| 1 | 1.5 |
2.7 |
2.75 |
96% |
| 1 | 0.8 |
1.9 |
1.98 |
94% |
Storage
The kit must be stored at 2–8 °C. The opened components can be stored for one week at 2–8 °C.
Usage
This assay is for in vitro research use only and is not for use in diagnostic procedures.
Resources
Summary References
- Zec I, Tislaric-Medenjak D, Megla ZB, Kucak I. Anti-Müllerian hormone: a unique biochemical marker of gonadal development and fertility in humans. Biochem Med (Zagreb). 2011;21(3):219-30. doi: 10.11613/bm.2011.031. PMID: 22420235.
- Broer SL, Broekmans FJ, Laven JS, Fauser BC. Anti-Müllerian hormone: ovarian reserve testing and its potential clinical implications. Hum Reprod Update. 2014 Sep-Oct;20(5):688-701. doi: 10.1093/humupd/dmu020. Epub 2014 May 12. PMID: 24821925.
- Moolhuijsen LME, Visser JA. Anti-Müllerian Hormone and Ovarian Reserve: Update on Assessing Ovarian Function. J Clin Endocrinol Metab. 2020 Nov 1;105(11):3361–73. doi: 10.1210/ clinem/dgaa513. PMID: 32770239; PMCID: PMC7486884.
Ilex Life Sciences LLC is an authorized distributor of PromedeusLab.