Ilex Life Sciences Matrix Gla Protein (MGP) Human, E. coli Recombinant Protein

Matrix Gla Protein (MGP) Human, E. coli Recombinant Protein

A1922020
$225.00
$225.00
$267.00
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Description

Matrix Gla Protein (MGP) Human, E. coli Recombinant Protein is a single, non-glycosylated polypeptide chain containing 98 amino acids (20-96) and has a molecular mass of 11.8 kDa. The MGP is fused to a 20 amino acid His-Tag at N-terminus and is purified by proprietary chromatographic techniques.

Summary

Matrix Gla protein (MGP) is a member of a family of vitamin-K2 dependent, Gla-containing proteins. MGP has a high affinity binding to calcium ions, similar to other Gla-containing proteins. The protein acts as an inhibitor of vascular mineralization and plays a role in bone organization. MGP is vital for the regulation of calcification in the extracellular matrix, particularly in cartilage and arteries.

Other Names

Matrix Gla protein, Cell growth-inhibiting gene 36 protein, GIG36, MGLAP, NTI.

Application Notes

A study by Nishimoto S. K. & Nishimoto M. concluded that "cells attach and spread better on fibronectin and MGP coated surfaces than to fibronectin alone, even though MGP itself has no cell attachment activity." 

Fig. 1.: MGP augments cell spreading on fibronectin. The graph shows the calculated cell area/cell for fibronectin coated surfaces compared to fibronectin plus MGP coated surfaces.
Fig. 1.: MGP augments cell spreading on fibronectin. The graph shows the calculated cell area/cell for fibronectin coated surfaces compared to fibronectin plus MGP coated surfaces.

Source: Nishimoto, S. K., & Nishimoto, M. (2014). Matrix gla protein binds to fibronectin and enhances cell attachment and spreading on fibronectin. International journal of cell biology, 2014, 807013. https://doi.org/10.1155/2014/807013

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MYESHESMES YELNPFINRR NANTFISPQQ RWRAKVQERI RERSKPVHEL NREACDDYRL CERYAMVYGY NAAYNRYF

Type

Recombinant Protein

Source

E. coli

Purity

Greater than 90%.

Formulation

Sterile-filtered colorless solution. The MGP solution (1 mg/ml) contains 20mM Tris-HCl buffer (pH 8.0) and 10% glycerol.

Shipping

Shipped on regular ice packs. Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.

Usage

This product is intended for Laboratory Research Use Only. Not for use in diagnostic or therapeutic procedures. This product may not be used as a pharmaceutical or veterinary drug, agricultural product, or food additive.

RESOURCES

Want to learn more about MGP? We have compiled the links below which contain information that you may find interesting.

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