Description
Macrophage Colony-Stimulating Factor 1 (M-CSF) Human, His Tag, E. coli Recombinant Protein is a single, non-glycosylated, polypeptide chain containing 179 amino acids (33-190 a.a.) and has a total molecular mass of 20.7 kDa. The M-CSF is fused to a 20 amino acid His Tag at N-terminus and is purified by proprietary chromatographic techniques.
Summary
Macrophage colony-stimulating factor (M-CSF) is a cytokine that plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. Required for normal male and female fertility. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration. Plays a role in lipoprotein clearance. [Reference: UniProtKB - A0A024R0A1 (CSF1_HUMAN)].
Other Names
His-Tagged M-CSF, Human M-CSF, CSF-1, CSF1 gene product, MCSF
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MEEVSEYCSH MIGSGHLQSL QRLIDSQMET SCQITFEFVD QEQLKDPVCY LKKAFLLVQD IMEDTMRFRD NTPNAIAIVQ LQELSLRLKS CFTKDYEEHD KACVRTFYET PLQLLEKVKN VFNETKNLLD KDWNIFSKNC NNSFAECSSQ DVVTKPDCN.
Source
E. coli
Formulation
Sterile filtered colorless solution. The M-CSF protein solution contains 20mM Tris-HCl, pH-8, 2mM DTT, and 10% Glycerol.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Shipping
Shipped on regular ice packs. Upon receipt, store the product at the temperature recommended below.
Storage/Expiration
Store at 4°C if entire vial will be used within 2-4 weeks. For longer periods of time, store frozen at -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Usage
This product is intended for Laboratory Research Use Only. Not for use in diagnostic or therapeutic procedures. This product may not be used as a pharmaceutical or veterinary drug, agricultural product, or food additive.
Resources
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