Description
Our bioactive FGF-2 (FGF-basic) Bovine, E. coli Recombinant Protein (147 aa), also known as truncated Fibroblast Growth Factor 2, is a single, non-glycosylated, polypeptide chain that contains 147 amino acids and has a molecular mass of approximately 16.5 kDa. The FGF-2 is purified by proprietary chromatographic techniques.
Summary
FGF-basic, a member of the fibroblast growth factor (FGF) family, is a non-glycosylated, heparin-binding growth factor and signaling protein encoded by the FGF2 gene. It binds to and exerts effects via specific fibroblast growth factor receptor (FGFR) proteins. Like other FGF family members, FGF-basic possesses broad mitogenic and cell survival activities, and is involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion.
Additionally, FGF-basic is a critical component of human embryonic stem cell (hESCs) culture medium; the growth factor is necessary for the cells to remain in an undifferentiated state. As a result, FGF-basic has been used to develop chemically-defined, serum-free and feeder-free hESCs culture mediums.
Other Names
Truncated bovine Fibroblast Growth Factor-2, Cow FGF2, FGF-2, FGF basic, FGF-b, Basic fibroblast growth factor, bFGF, Heparin-binding growth factor 2, HBGF-2.
Amino Acid Sequence
MPALPEDGGS GAFPPGHFKD PKRLYCKNGG FFLRIHPDGR VDGVREKSDP HIKLQLQAEE RGVVSIKGVC ANRYLAMKED GRLLASKCVT DECFFFERLE SNNYNTYRSR KYSSWYVALK RTGQYKLGPK TGPGQKAILF LPMSAKS.
Source
E. coli
Formulation
Sterile filtered white lyophilized (freeze-dried) powder. The protein was lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
Purity
Purity greater than 97.0% as determined by:
- Analysis by RP-HPLC.
- Analysis by SDS-PAGE.
Biological Activity
The ED50 as determined by a cell proliferation assay using murine balb/c 3T3 cells is < 0.1 ng/ml, corresponding to a specific activity of > 1.0 ×107IU/mg.
Reconstitution
It is recommended to reconstitute the lyophilized FGF-basic in sterile PBS not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Shipping
At ambient temperature. Upon receipt, store the product at the temperature recommended below.
Storage/Expiration
Lyophilized FGF-basic, although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution, FGF-basic should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Usage
This product is intended for Laboratory Research Use Only. Not for use in diagnostic or therapeutic procedures. This product may not be used as a pharmaceutical or veterinary drug, agricultural product, or food additive.
Resources
Want to learn more about FGF-basic? We have compiled the links below which contain information that you may find interesting.
- UniProt Knowledgebase: UniProtKB - P03969 (FGF2_BOVINE)
- National Library of Medicine: Gene ID: 281161: FGF2 fibroblast growth factor 2 [Bos taurus (cattle)]