Description
3-D Life PEG-Link is a component of the 3-D Life Hydrogel system. The PEG-Link molecule consists of polyethylene glycol carrying a thiol at each end. When combined with polymers of the 3-D Life Hydrogel system, thiol groups on PEG-Link form stable thioether bonds with the thiol-reactive groups on the polymers, which results in the formation of the hydrogel.
PEG-Link can be used with 3-D Life functionalized polymers Mal-PVA, Mal-Dextran, SG-PVA and SG-Dextran.
For using this product please consult General Protocols GP-1 "Preparation of 3-D Life Fast Gelling Hydrogels" or GP-2 "Preparation of 3-D Life Slow Gelling Hydrogels" and the 3-D Life Hydrogels User Guide, which may be downloaded below.
Application
3-D Life PEG-Link is used together with thiol-reactive polymers of the 3-D Life Hydrogel system to establish 3-D cell cultures.
Quantity
Cat. No. L50-1: When used with polymers of the 3-D Life Hydrogel system up to 2 ml 3-D Life Hydrogel can be generated, depending on the stiffness of the gel.
Cat. No. L50-3: When used with polymers of the 3-D Life Hydrogel system up to 6 ml 3-D Life Hydrogel can be generated, depending on the stiffness of the gel.
Components
Cat. No. L50-1:
| Material | Quantity | Concentration of reactive groups | |
|---|---|---|---|
| PEG-Link, Lyophilized |
200 µl* | 20 mmol/L* | MSDS |
| Water | 600 µl | n.a. | MSDS |
| *Volume after reconstitution of lyophilisate. |
*Concentration after reconstitution of lyophilisate. |
Cat. No. L50-3:
| Material | Quantity | Concentration of reactive groups | |
|---|---|---|---|
| PEG-Link, Lyophilized | 3 x 200 µl* | 20 mmol/L* | MSDS |
| Water | 600 µl | n.a. | MSDS |
| *Volume after reconstitution of lyophilisate. | *Concentration after reconstitution of lyophilisate. |
Resources
- Product Datasheet: 3-D Life PEG-Link (200 µl)
- Product Datasheet: 3-D Life PEG-Link (3x 200 µl)
- Protocol: Preparation of 3-D Life Fast (FG) Gelling Hydrogels
- Protocol: Preparation of 3-D Life Slow Gelling (SG) Hydrogels
- 3-D Life Hydrogel User Guide
- 3-D Life Biomimetic Hydrogels Page
- 3-D Life Hydrogels User Applications
- 3-D Life Hydrogels Product References
Application Notes
- Application Note: Epithelial Cyst Formation in 3-D Life Hydrogels
- Application Protocol: MDCK Cyst Formation in 3-D Life Hydrogels
- Application Note: Fibroblast Spreading in Cell-Degradable 3-D Life Hydrogels
Ilex Life Sciences LLC is an authorized distributor of Cellendes GmbH.