Description
The 3-D Life Dextran-HA Hydrogel Kit provides reagents for setting up hydrogels containing hyaluronic acid (hyaluronan).
Its major components are SG-Dextran and the thiol-functionalized hyaluronic acid crosslinker HyLink. When SG-Dextran and HyLink are combined, thiol groups on HyLink form stable thioether bonds with thiol-reactive groups on SG-Dextran, which results in the formation of the gel. The components are mixed at physiological pH (pH 7.2) for optimal cell compatibility. The slow gelation kinetics allows enough time to conveniently manipulate the solution before the onset of gel formation. The hydrogel allows cell spreading and migration if cell adhesion peptides are present in the gel.
Prior to the crosslinking step, cell adhesion peptides (e.g. 3-D Life RGD Peptide, Cat. No. 09-P-001) can be covalently attached to a portion of the SH-reactive groups on SG-Dextran to provide a cell-adhesive matrix.
Dextran hydrogels can be dissolved by the addition of dextranase (3-D Life Dextranase, Cat. No. D10-1), which allows the recovery of chemically fixed or live cells for post-culture analyses (e.g. RT-PCR) or for further cultivation.
For more information and instructions, please consult the General Protocol GP-4 "Preparation of 3-D Life Hyaluronic Acid (HA) Hydrogels" and the 3-D Life Hydrogels User Guide, which may be downloaded below.
Application
3-D cell culture, hydrogel injections, filling of microchannels, generation of soft to very stiff gels.
Quantity
Allows formation of 3.3 ml 3-D Life Hydrogel of a crosslinking strength of 1.2 mmol/L.
Components
| Material | Quantity | Concentration of reactive groups | |
|---|---|---|---|
| SG-Dextran |
170 µl | 30 mmol/L | MSDS |
| HyLink, lyophilized | 2 x 200 µl* | 10 mmol/L* | MSDS |
| 10x CB Buffer (pH 7.2) | 200 µl | n.a. | MSDS |
| Water | 2 x 1500 µl | n.a. | MSDS |
| *Volume/concentration after reconstitution of lyophilisate. | |||
Resources
- Product Datasheet: 3-D Life Dextran-HA Hydrogel SG Kit
- Protocol: Preparation of 3-D Life Hyaluronic Acid (HA) Hydrogels
- Protocol: Degradation of dextran-based hydrogels with dextranase
- 3-D Life Hydrogel User Guide
- 3-D Life Biomimetic Hydrogels Page
- 3-D Life Hydrogels User Applications
- 3-D Life Hydrogels Product References
Ilex Life Sciences LLC is an authorized distributor of Cellendes GmbH.